Journal: Dentistry journal

Article Title: Evaluation of Two Configurations of Hydroxyapatite and Beta-Tricalcium Phosphate in Sinus Grafts with Simultaneous Implant Installation: An Experimental Study in Rabbits.

doi: 10.3390/dj11050121

Figure Lengend Snippet: Figure 9. Photomicrographs of the maxillary sinus region of rabbits 40 days after filling with HA + β-TCP in the “paste and granule” configurations and concomitant implant installation, showing immunolabeling of RUNX2, VEGF, OCN, and TRAP proteins. The black arrows indicate examples of immunolabeling.

Article Snippet: The primary antibodies used were polyclonal antibodies, produced in goats, against RUNX2 (SC8566), VEGF (SC1881), OCN (SC18319) and TRAP (SC 30832) (Santa Cruz Biotechnology, Santa Cruz, CA, USA).

Techniques: Immunolabeling

Journal: FEBS Open Bio

Article Title: In vitro evaluation of periapical lesion‐derived stem cells for dental pulp tissue engineering

doi: 10.1002/2211-5463.13336

Figure Lengend Snippet: Primers used in the qRT‐PCR.

Article Snippet: The membranes were blocked with 5% non‐fat milk and incubated with primary antibodies against RUNX family transcription factor 2 (RUNX2) (dilution 1 : 500; #12556S; Cell Signaling Technology, Danvers, MA, USA), dentin sialophosphoprotein (DSPP) (dilution 1 : 500; sc‐73632, Santa Cruz Biotechnology, Santa Cruz, CA, USA), dentin matrix protein 1 (DMP1) (dilution 1 : 500; ab103203; Abcam, Cambridge, UK), Sp7 transcription factor (SP7) (dilution 1 : 1000; PA5‐115697; Thermo Fisher), VEGF (dilution 1 : 100; MA5‐13182; Thermo Fisher), GDNF (dilution 1 : 200; 711074; Thermo Fisher) and GAPDH (WB0197; Biotechwell) and were then incubated with HRP‐conjugated secondary antibodies (dilution 1 : 5000; Biotechwell).

Techniques: Sequencing

Journal: FEBS Open Bio

Article Title: In vitro evaluation of periapical lesion‐derived stem cells for dental pulp tissue engineering

doi: 10.1002/2211-5463.13336

Figure Lengend Snippet: Comparison of the osteo/odontogenic capacities of the cells. (A) ALP staining after osteo/odontogenic induction for 3 and 7 days. Scale bar = 500 μm. (B) Alizarin red staining showing the presence of calcified nodules after 7, 14, 21 and 28 days of osteo/odontogenic induction. Scale bar = 500 μm. (C) Gene expression patterns of ALP , RUNX2 and DSPP after osteogenic induction for 3 and 7 days; the expression level was normalized to that of ACTB ( n = 3, Student’s t ‐test). * P < 0.05, ** P < 0.01. (D) Western blotting detection (upper) and imagej analysis (below) of DSPP, DMP1, RUNX2 and SP7 after osteogenic induction for 7, 14 and 21 days. Data are shown as the mean ± SD.

Article Snippet: The membranes were blocked with 5% non‐fat milk and incubated with primary antibodies against RUNX family transcription factor 2 (RUNX2) (dilution 1 : 500; #12556S; Cell Signaling Technology, Danvers, MA, USA), dentin sialophosphoprotein (DSPP) (dilution 1 : 500; sc‐73632, Santa Cruz Biotechnology, Santa Cruz, CA, USA), dentin matrix protein 1 (DMP1) (dilution 1 : 500; ab103203; Abcam, Cambridge, UK), Sp7 transcription factor (SP7) (dilution 1 : 1000; PA5‐115697; Thermo Fisher), VEGF (dilution 1 : 100; MA5‐13182; Thermo Fisher), GDNF (dilution 1 : 200; 711074; Thermo Fisher) and GAPDH (WB0197; Biotechwell) and were then incubated with HRP‐conjugated secondary antibodies (dilution 1 : 5000; Biotechwell).

Techniques: Comparison, Staining, Gene Expression, Expressing, Western Blot

Journal: Bioengineering

Article Title: An Injectable, Osteoconductive Gelatin-Enabled GelMA/HAp Hydrogel Scaffold for Minimally Invasive Bone Tissue Engineering

doi: 10.3390/bioengineering13020139

Figure Lengend Snippet: ( a ) Immunofluorescence images of Runx2 in BMSCs co-cultured with hydrogels during osteogenic differentiation. ( b ) Number of Runx2-positive cells in BMSCs co-cultured with hydrogels. *** p < 0.005; **** p < 0.0001.

Article Snippet: The fixed samples were permeabilized and blocked, followed by incubation with a primary antibody against Runx2 (Shanghai, China, Proteintech, 20700-1-AP) and then with the corresponding fluorophore-conjugated secondary antibody (USA, Thermo Fisher Scientific, A-11011).

Techniques: Immunofluorescence, Cell Culture

Journal: Bioengineering

Article Title: An Injectable, Osteoconductive Gelatin-Enabled GelMA/HAp Hydrogel Scaffold for Minimally Invasive Bone Tissue Engineering

doi: 10.3390/bioengineering13020139

Figure Lengend Snippet: ( a – d ) qPCR analysis of OCN , COL-1 , Runx2 and Osterix in BMSCs on Day 7 and 14. * p < 0.05; **** p < 0.0001.

Article Snippet: The fixed samples were permeabilized and blocked, followed by incubation with a primary antibody against Runx2 (Shanghai, China, Proteintech, 20700-1-AP) and then with the corresponding fluorophore-conjugated secondary antibody (USA, Thermo Fisher Scientific, A-11011).

Techniques:

Journal: International Journal of Nanomedicine

Article Title: Anti-Infection Efficacy, Osteogenesis Potential, and Biocompatibility of 3D Printed PLGA/Nano-Hydroxyapatite Porous Scaffolds Grafted with Vancomycin/DOPA/rhBMP-2 in Infected Rabbit Bone Defects

doi: 10.2147/IJN.S514978

Figure Lengend Snippet: Immunohistochemical analysis of osteogenesis-related proteins in IBD rabbit models. ( A ) Representative immunohistochemical staining images showing RUNX2 and OCN expression in bone tissues of IBD rabbit models after scaffold implantation at 12 weeks post-surgery. ( B and C ) Quantification of RUNX2-positive and OCN-positive cells in each group. **p < 0.01, ***p < 0.001 versus the PLGA/n-HA group; ### p < 0.001 versus the PLGA/n-HA/VAN group.

Article Snippet: After 1 h of non-specific binding blockage with 10% goat serum, the sections were incubated with the primary antibodies against RUNX2 (#A2851; 1:100; ABclonal, Wuhan, China) and OCN (#HZK-11654; 1:100; Huzhen, Shanghai, China) at 4 °C overnight and washed three times with PBS.

Techniques: Immunohistochemical staining, Staining, Expressing

Journal: Stem Cells (Dayton, Ohio)

Article Title: LncRNA‐OG Promotes the Osteogenic Differentiation of Bone Marrow‐Derived Mesenchymal Stem Cells Under the Regulation of hnRNPK

doi: 10.1002/stem.2937

Figure Lengend Snippet: Osteogenesis‐associated long noncoding RNA (lncRNA‐OG) promotes the osteogenic differentiation of bone marrow‐derived mesenchymal stem cells (BM‐MSCs). (A): Left: alkaline phosphatase (ALP) staining on day 7 (upper panel), alizarin red (ARS) staining on day 14 after osteogenic induction (lower panel). Right: ALP activity was determined as units per gram of protein per 15 minutes. ARS staining was quantified as the absorbance at 562 nm. (B): Relative mRNA levels of RUNX2 and ALP detected by quantitative real‐time polymerase chain reaction (qRT‐PCR) on day 7 of osteogenic differentiation. Relative mRNA levels of OSX and OCN detected by qRT‐PCR on day 10 of osteogenic differentiation. Data were normalized to GAPDH expression. (C): Western blotting analysis of RUNX2 protein levels on day 7 of osteogenic induction. GAPDH was used as the internal control (upper panel). Lower, quantification of band intensities (lower panel). (D): H&E staining, Masson's trichrome staining, and immunohistochemical staining of OCN in lncRNA‐OG and NC groups. Scale bars, 100 μm. Data are presented as the mean ± SD. *, p < .05; **, p < .01 ( n = 3 independent experiments). See also Supporting Information Figure .

Article Snippet: Primary antibodies against RUNX2 (8486; Cell Signaling Technology [CST], Danvers, MA), total β‐catenin (8480; CST), active β‐catenin (19807; CST), ERK1/2 (4695; CST), phospho‐ERK1/2 (4370; CST), hnRNPK (ab39975; Abcam, Cambridge, U.K.), smad1 (6944; CST), phospho‐smad1/5/8 (13820; CST), BMP2 (ab14933; Abcam), BMP4 (ab124715; Abcam), BMP6 (ab155963; Abcam), BMP7 (ab129156; Abcam), BMP9 (ab207318; Abcam), β‐actin (3700; CST), and GAPDH (ab8245; Abcam) were diluted 1:1,000 and incubated with the membranes at 4°C overnight.

Techniques: Derivative Assay, Staining, Activity Assay, Real-time Polymerase Chain Reaction, Quantitative RT-PCR, Expressing, Western Blot, Control, Immunohistochemical staining